competent cell preparation

Ted Michelini tedm at
Tue Oct 31 01:45:59 EST 1995

In article <Pine.3.89.9510300030.A538975791-0100000 at SKYCAT.USask.CA>,
lees at SASK.USASK.CA wrote:

> A while ago, there was a discussion on SIMPLE & EASY & RELIABLE method of 
> bacterial competent cell preparation. I'm looking for that publication. I 
> remember that it used PEG, Glycerol, LB media as storage solution,  and 
> may have been published in PNAS but I'm not so sure.
> It'll be appreciated if anyone get me information on this matter.
> Thanks very much,
> Lee

   The other post has had luck with this but in my lab the transformation
was 10-100 fold off simple CaCl2 transformation...Why'd they publish in
PNAS anyway?


Ted Michelini
Institute of Molecular Biology
University of Oregon

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