competent cell preparation
howe at DARWIN.UCSC.EDU
Tue Oct 31 14:39:44 EST 1995
I believe the reference you're looking for is:
C.T. Chung et al (1989)
PNAS USA V86:2172-2175
One step preparation of competent E. coli: Transformation and storage
of bacterial cells in the same solution.
My advisor keeps pushing me to try this method, but I just haven't had
the time to do it (imagine that), so I can't lend any opinions on the
"The onions expressed here are my own"
On 31 Oct 1995, Ted Michelini wrote:
> Date: 31 Oct 1995 06:45:59 GMT
> From: Ted Michelini <tedm at darkwing.uoregon.edu>
> To: methods-and-reagents at net.bio.net
> Subject: Re: competent cell preparation
> In article <Pine.3.89.9510300030.A538975791-0100000 at SKYCAT.USask.CA>,
> lees at SASK.USASK.CA wrote:
> > A while ago, there was a discussion on SIMPLE & EASY & RELIABLE method of
> > bacterial competent cell preparation. I'm looking for that publication. I
> > remember that it used PEG, Glycerol, LB media as storage solution, and
> > may have been published in PNAS but I'm not so sure.
> > It'll be appreciated if anyone get me information on this matter.
> > Thanks very much,
> > Lee
> The other post has had luck with this but in my lab the transformation
> was 10-100 fold off simple CaCl2 transformation...Why'd they publish in
> PNAS anyway?
> Ted Michelini
> Institute of Molecular Biology
> University of Oregon
More information about the Methods