GST fusion protein--pl help
Tay Hou Ngee Agnes
mcbtayhn at leonis.nus.sg
Tue Oct 24 19:58:05 EST 1995
When I worked with GST fusion proteins and had an insoluble porduct I
tried adding 6M guanidinium hydrochloride and it worked well. Had to
dialyze off the guanidnium but at least I obtained enough protein to work
with. Not sure though if your protein will be adversely affected.
Have never tried the protease-def bugs as someone else tried them and
found no improvement for her proteins. If solubility is your problem
rather than degradation, I dont think you need to worry abt using
Hope this helps.
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