competent cell preparation

Ken Howe howe at DARWIN.UCSC.EDU
Tue Oct 31 19:35:39 EST 1995


Dear Netters,
That last message was sent by my advisor.  Evidently, there's someone in 
the lab here that turned me in.  My apologies.

Ken Howe
"The onions expressed here are those of my advisor"

On Tue, 31 Oct 1995 
sherif at darwin.ucsc.edu wrote:

> Date: Tue, 31 Oct 1995 13:09:05 -0800 (PST)
> From: sherif at darwin.ucsc.edu
> To: Ken Howe <howe at darwin.ucsc.edu>
> Cc: methods-and-reagents at net.bio.net
> Subject: Re: competent cell preparation
> 
> Ken,
> How can you possibly have time to look up references for other people?
> Get away from that computer and get back to work!!
> Manny
> 
> On 31 Oct 1995, Ken Howe wrote:
> 
> > I believe the reference you're looking for is:
> >   C.T. Chung et al (1989)
> >   PNAS USA V86:2172-2175
> >   One step preparation of competent E. coli: Transformation and storage
> >   of bacterial cells in the same solution.
> > 
> > My advisor keeps pushing me to try this method, but I just haven't had 
> > the time to do it (imagine that), so I can't lend any opinions on the 
> > matter.
> > 
> > Ken Howe
> > "The onions expressed here are my own"
> > 
> > On 31 Oct 1995, Ted Michelini wrote:
> > 
> > > Date: 31 Oct 1995 06:45:59 GMT
> > > From: Ted Michelini <tedm at darkwing.uoregon.edu>
> > > To: methods-and-reagents at net.bio.net
> > > Subject: Re: competent cell preparation
> > > 
> > > In article <Pine.3.89.9510300030.A538975791-0100000 at SKYCAT.USask.CA>,
> > > lees at SASK.USASK.CA wrote:
> > > 
> > > > A while ago, there was a discussion on SIMPLE & EASY & RELIABLE method of 
> > > > bacterial competent cell preparation. I'm looking for that publication. I 
> > > > remember that it used PEG, Glycerol, LB media as storage solution,  and 
> > > > may have been published in PNAS but I'm not so sure.
> > > > 
> > > > It'll be appreciated if anyone get me information on this matter.
> > > > 
> > > > 
> > > > Thanks very much,
> > > > 
> > > > Lee
> > > 
> > > Lee,
> > >    The other post has had luck with this but in my lab the transformation
> > > was 10-100 fold off simple CaCl2 transformation...Why'd they publish in
> > > PNAS anyway?
> > > 
> > > regards,
> > > 
> > > Ted Michelini
> > > Institute of Molecular Biology
> > > University of Oregon
> > > 
> > > 
> > 
> > 
> 



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