Alternatives to Ethidium Bromide

M.Alexeyev Malexeyev at biost1.thi.tmc.edu
Tue Oct 31 17:21:28 EST 1995


In article <pnorton-0111950356090001 at norton1.jmc.tju.edu>,
pnorton at lac.jci.tju.edu (Pamela Norton) wrote:

> In article <1100 at kiwi.ukc.ac.uk>, gwp1 at ukc.ac.uk (G.W.Phillips) wrote:
> 
> > we are currently seeking a n alternative method for visualizing DNA in
agarose
> > gels. Can anyone suggest a cheap and safe alternative to ethidium bromide.
> > gels. Can anyone suggest an alternative to ethidium bromide which is
not toxic
> > and reasonably inexpensive.
> > 
> 
> 
>    What about methylene blue? Can anyone comment on the sensitivity and
> cost for  routine use?
> 

I wasn't overly impressed with sensitivity of methylene blue staining. It
is OK for preparative gels, but that is about all it is good for (in my
book). I would like to hear about positive impressions,too.

M.A.



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