Need killer gene

jim hartley jhartley at
Tue Oct 31 06:21:24 EST 1995

Can anyone suggest a gene deadly to most E. coli cloning strains, that 
can be propagated in a particular mutant?  ccdB would be great, but I 
can't get the gyrA462 mutant because of patents.  Streptomycin 
sensitivity is OK if it kills completely enough, but I'd rather avoid the 
necessity of including strep in all my plates.  

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