Methods October 1995 Archives by thread
Starting: Sun Oct 1 00:36:54 EST 1995
Ending: Tue Oct 31 23:11:17 EST 1995
Messages: 1364
- imaging software
Alan Silverman
- S1 nuclease
Vicki Burkitt
- E-box binding bHLH protein antibody search
David LeBrun
- Gustatory library
Mike Witty
- thermosequenase
Karl Voss
- RNAse protection assay on multiple samples
nobody at nowhere
- "recycling" Qiagen columns
Hamish Scott
- Source for 9E10 Ab?
Tom Anderson
- differential molecular screening
Lely Chow
- cyclophilin
rey garcia
- cAMP ASSAY
Steve Mcclue
- MUltiple sequence Alignment Software
Peter Rice
- Beta-gal Non lethal Assays???
Michael Strehle
- NAOMI Version 2.2
Simon M. Brocklehurst
- Differential display shortcomings?
Maura J Meade
- BRL AFLP kit
Chris Jones
- Commercial sequencing primers
baculo at rcl.wau.nl
- Transfection of AG-8 cells (lymphocytes)
Volker Blaschke
- 10X TBS
Joe Stains
- Fisher Semi dry blotter
Joe Stains
- Long PCR Questions
Harry Nickla
- Help!!!Gel box cause for RNA degradation???
Robert Horton
- Home-Made TA cloning
JTILL
- Removal of glycogen from DNA
Robert Horton
- gel scanning software?
Greg Appleyard
- MUltiple sequence Alignment Software
Robert Horton
- Which exonuclease can cut a 5' overhang?
Laurentiu COCEA
- ethidium bromide alternative?
Robert Horton
- Transfection of AG-8 cells (lymphocytes)
Dima Klenchin
- ethidium bromide alternative?
SCHNEE at
- dam - bacterial strain
Paul N Hengen
- Fluorescent free fatty acid probe/ADIFAB
res-shh at inet.uni-c.dk
- Nuserum Shortage
Jeanmarie Donovan
- ethidium bromide alternative?
K.G. Korth
- Ni-NTA column and internal poly-His stretch
QIAGEN
- Killing Taq
David L. Haviland, Ph.D.
- Help on Transilluminators
To: Nikolai
- Manufacturer of MolecuSol detergent?
Matthew Bowen Harrington
- cycloheximide & muscle primary cultures
"joseph john leddy ", grad stud
- In Situ Cell Death
Diana07
- Multichannel Pipettes
Brad Thomas
- prokaryotic mRNA in frozen tissues or fluids
SHILKA
- nucleic acid labeling and detection
Joan Boyce
- Proliferation assay needed
To: Nikolai
- Mol Biologists, do any of you use precast gels?
Paul D. Boyer
- 5' RACE-Tail cDNA with dC, or dT?
Garry P. Larson
- BIOSCI address database entry form now at http://www.bio.net/
BIOSCI Administrator
- Qiagen buffers
Ng Wei Pern Patrick
- Sheep mitochondrial DNA sequence
Dr Jim Cummins
- ABI automated sequencer
Dale Levitzke
- Dihydrofolate synthesis?
Fourie Joubert
- SDS-PAGE
R. Woodward
- dNTPs can inhibit (diffential displ.)RT-PCR
VOGEL at ubaclu.unibas.ch
- Determining counting efficiency of Scint. Counter for 32P
Mary P. Remington
- looking for plasmids
Patrick Van Dijck
- looking for plasmids
Patrick Van Dijck
- Help with pcr direct from colonies
mdjlsc at cc.newcastle.edu.au
- Commercial source of cre protein
John Dixon
- Help: denaturing size standards
Peter Kaplan
- help with poly(A)+ mRNA purification
Anna-Maria
- Ni-NTA column and internal poly-His stretch.
Ian Clarke
- Cre-lox etc...
Przemko
- Apoptosis induction in murine fibroblasts
John Dixon
- looking for recombinant plasminogen
Estanis Navarro G¢mez
- methyltransferase activity
Juan A. Ayala
- Help: bacteriophage P1 vectors
Paquette Yves
- Lambda terminase
Jose E. Mejia
- Sma I digestion of genomic DNA
Shin Enomoto
- Purchasing from storerooms
Bioinfo
- GST Plasmids
Colin Rasmussen
- cDNA synthesis > 6kb
Mr. S.J. Ennion
- Stability of Gene Construction kit
Chris Ferrer
- Commercial source for deoxy-Inosine CED phosphoramidite
natalie unruh
- DNA column purifications
Paul N Hengen
- ethidium bromide alternative?
Brad Thomas
- Electro-blotting DNA
Brad Thomas
- PCR problem
Edward H. Wang
- What' the real length of normal genomic DNA
Greg Smith
- transform bacteria with linear DNA?
kang at msvax.mssm.edu
- gel-shift problem
xiaokezh at kuhub.cc.ukans.edu
- BIOSCI node at net.bio.net is DOWN!
BIOSCI Administrator
- Spectrophotometer
MICROPLATE
- P1 phage/ plasmid query
Stephen Perry
- test don't read
CDMENU at ccs1.cc.monash.edu.au
- test-don't read
CDMENU at ccs1.cc.monash.edu.au
- ECL Optimisation
Adam Boyt
- Northern Blotting problem
Henry the Hungry Hippo
- Commercial sequencing primers
COLINE at rulsfb.LeidenUniv.nl
- Rat & Murine IL-5 and IL-4 PCR Primers
HanNibal
- Storing Northerns
Tracy Aquilla
- computer program for finding promoters
samir bhagwat
- 6-His Ab/accessing archives
Gaughan
- Stability of recom. antibodies?
Peter
- dual mamallian expression vector?
Grtful ned
- RACE
3wsm1 at qlink.queensu.ca
- LPA ppt in culture media
Rene Mauricio Salazar
- RNA transferring
Abby Qiu
- Hot Probe by asymmetric PCR
Michael J. Tuvim
- BL21 (DE3) Induction
Michael J. Tuvim
- FACS and yeast
Daniel
- protein precipitation
Tomas Drgon
- BIOSCI node at net.bio.net is DOWN!
David Kristofferson
- Alpha-amylase anyone?
Nick C.K. Heng
- gel-shift problem
Eric Verdin
- gel-shift problem
Eric Verdin
- Source for plastic toothpicks
Bob Kodrzycki
- Hot probe by asymmetric Pcr
Andrei Popov
- biological grinding spheres???
MDELAVINA at IRRI.CGNET.COM
- HELP WITH RESEARCH ABOUT BIO-CHEMISTRY AS A PROFESSION
Async User
- Immunology - mitogenic antibodies
deepa at aruba.ccit.arizona.edu
- What' the real length of normal genomic DNA
oRutebuka
- CD-ROM to demonstrate molecular techniques for undergraduates and postgraduates
Robert Coelen
- Using Glyoxal for RNA gel electrophoresis
bruno Sargueil
- Promega RT-PCR
David Tak Yan Yu
- help! Differential Display Trouble
ssd2rc at po.iijnet.or.jp
- CNBR Digestion on SDS-PAGE gels Slices??
Dan Roberts
- Baculo plaque test ?
Burkhard Hassel
- Looking for Neeraj Jain in Singapore!
Ian Clarke
- Long Ranger Gel Problem
Steve Whitwill
- ligation problems and electroporation
Bob Kodrzycki
- help! Differential Display Trouble
Don Haut
- transform bacteria with linear DNA?
Hank Seifert
- dna sequencing
sm0j at Lehigh.EDU
- deleted postings
Gaughan
- pCITE vector sequence
Dr. P.A. de Zoysa
- large scale CHO cells cultivation ????
Corinne Belville
- LPA ppt in culture media
Rene Mauricio Salazar
- Change in LB recipe?
Dale Yuzuki's Work Account
- MABs against mouse MLH1, PMS1, PMS2, REP3
Laurentiu COCEA
- dna sequencing
Sasha Kraev
- Mol Biologists, do any of you use precast gels?
Anton Hutticher
- Trimming 5' protrusive ends?
Paul E. Ulanch
- GeneRunner or equivalent
Todd Johnson
- large scale CHO cells cultivation ????
Hugh Goldie
- Pharmacia C Columns
Hugh Goldie
- Help!!!Gel box cause for RNA degradation???
Kenneth Y. Ilio
- Polynucleotide Kinase At pH 9.5
Hugh Goldie
- Anybody getting unsolicited e-mail?
K.G. Korth
- Kinasing a probe with T4 polynucleotide kinase
Jim Smith
- Kinasing a probe with T4 polynucleotide kinase
Jim Smith
- pCITE vector sequence
Ian Clarke
- (no subject)
Mark Naylor
- gel-shift problem
Sergey V. Orlov
- PCR mutagenesis
Andy Kravetz
- Help !- tRNA cont. of DNA
Harold Drabkin
- purification of 100 bp fragments
Eric C. Anderson
- Affinity column for protein purification
G.P.
- Q: Southern Hyb times
scalzod at fred.fhcrc.org
- expression vector with Kan selection
Anthony J. Persechini
- transform bacteria with linear DNA?
Dr. P.A. de Zoysa
- inverse PCR
Kevin Lease
- Cleaving GST-fusion proteins
aw at dna.bio.warwick.ac.uk
- Best T4 Ligase
SCComella
- Transfection Method, Rat Hepatocytes
SCComella
- PCR fragments sequencing
Jérôme Guerlotté
- expression vector with Kan selection
- mini-preps: NOT kits.
David L. Haviland, Ph.D.
- 5' RACE reaction reproducibility
Karen Peck-Miller
- pansorbin
Tomas Drgon
- Mutation rate in M13
benedik at uh.edu
- BIOSCI computer net.bio.net down Saturday for backups
BIOSCI Administrator
- Non-Radioactive In Situ Protocols
Andrew K. Groves
- PCR with 7-deaza analog
Jeff Palas
- Best T4 Ligase
Margon Vandongen
- Transfection Method, Rat Hepatocytes
Margon Vandongen
- Antibodies to mitotic cells or other cell cycle phases
Dave Bates
- library needed!
Benke/Belmar Login
- standard
Al
- Mung Bean Nuclease: Specific Activity?
Namjin Chung
- Gram postive marker
Rinkei Ko
- mac prgram for creating silent mutations
Doug Eernisse
- half life of Dynal magnetic beads?
Doug Eernisse
- Tritiated Thymidine and The Rate of DNA Syn.
Ronald P. Herzig, Jr.
- Bushnell-Haas Media??
Felicia M. Tomasko
- RAPD and capillary electrophoresis
bbadd at zedat.fu-berlin.de
- Sakata America, seed company
lees at SASK.USASK.CA
- DNA degradation
CDMENU at ccs1.cc.monash.edu.au
- purification of 100 bp fragments
Margon Vandongen
- Mutator strains of E. coli
R Daines
- Help on American Bioclinical
garrell
- An Invitrogen pcDNA3 question
Taymar Hartman
- Preparative gel retention for protein affinity purification
The Wagner Lab
- cDNA library
Seth Corey
- purification of 100 bp fragments
Jim Voeller
- E.coli anaerobical growth
Anatoly Sharipo
- actin as internal standard in RT-PCR
JIrving
- RNA ppt for Northerns
Denni Schnapp
- molecular marker mutation
Marjorie C Gurganus
- Difficult to clone
Xingnian Gu
- Research Fellowship Vacancy
Cathie Stewart-Moore
- what is it PEPSCAN?
V.Tchistiakov
- Rf: To boil or not to boil?
LOGAND
- Nucleotide triphosphates
Duncan Clark
- tat western blots
Mr P. Murphy
- Electroporation
Matthias Vogel
- electroporation
Matthias Vogel
- blue white screen
Georg J Seifert
- Protocols for isolation of plant proteins?
George F Edick
- PET Vectors expressing constitutively
ME. Homer
- Freezer Down - Is Taq still good?
NICHOLAS THEODORAKIS
- ECL optimisation
Glenn Cantor
- Freezer Down - Is Taq still good?
Jody K. Hirsh
- LKC media/broth for phiX174
mcgraw at bscr.uga.edu
- seeking T7 promoter/polymerase for eukaryotic system
Marion Freistadt
- lambda EMBL4 sequence
Jeffrey D. Bray
- Apoptosis
Diana07
- Gel software
workshop at dnatech.com
- Preparation of Lambda DNA
Gerald B. Downes
- (no subject)
R.D.Johnson
- L - cis - Diltiazem
~i~
- Thermostable RT
Robyn Wallace
- Direct sequencing from Lambda?
R.D.Johnson
- celltech e-mail address
gen at MED1.BMI.AC.CN
- Mounting Medium for Whole Mounts?
bfox at hooked.net
- Q:need reference for cDNA library consruction
Yi, Xiaoming
- CGH probe DNA concentration
prodley at chmeds.ac.nz
- Linear acrylamide method
Tim Dunn
- Seqence of Rat's Keratins (badly wanted)
Andrey Mikhailow
- Racks for Universals
MP. Thomas
- problem with immunoaffinity column
baudouin
- Primer Extension analysis. How?
jene
- Who Makes C-Tabb?
David Coleman
- RNA ppt, thanks!
Denni Schnapp
- Yeild of RNA from blood
M.C.Behrendt
- Who Makes C-Tabb?
Dima Klenchin
- Qiagen buffers
"Marianne Leverone ", BIO
- Qiagen buffers
M. Alexeyev
- Fragmentation of mouse IgG?
M.C.Behrendt
- Journal Impact Scores
Shaun Heaphy
- Freezer Down - Is Taq still good?
COLINE at rulsfb.LeidenUniv.nl
- Separating ssDNA from dsDNA (asymmetric PCR) ???
bmay
- (none)
"Gregory Rushing ", BIO
- Who Makes C-Tabb?
Tony Murray
- Cells secreting albumin
Sorin Damian
- Journal Impact Scores
dr._jack_komisar at WRSMTP-CCMAIL.ARMY.MIL
- Annealing ssDNA oligos. Is it easy?
Joaquim Culi
- finding T7 RNA pol gene and promoter of RNA pol I
Sergey V. Orlov
- Help with IgE
Melanie Simpson
- non-heat sterilization - suggestions?
Gregory Stuart
- non-heat sterilization - correction
Gregory Stuart
- finding T7 RNA pol gene and promoter of RNA pol I
M. Alexeyev
- Protocols for isolation of plant proteins?
bio.tamu.edu
- Sources for BHM71-18 mutS endA-
Greg
- Geneclean II Kit?
Hanbok Kim
- Agrobacterium problems
Steve Wylie
- Gentra Systems email or fax?
Tay Hou Ngee Agnes
- Sources of 8-oxoguanine
David Thomas
- non-heat sterilization - suggestions?
Gregory Stuart
- non-heat sterilization - suggestions?
Gregory Stuart
- DNA purification from Agarose (Sigma)
Hanbok Kim
- Tyrode buffer Ca++ deficient
E.vanBreda at FYS.RULIMBURG.NL
- Pre-filter recommendations?
Mad Dan Eccles
- Krebs Ringer phosphate medium
Victor Levitsky
- Pre-filter recommendations?
mbro at seqnet.dl.ac.uk
- Pre-filter recommendations?
mbro at seqnet.dl.ac.uk
- Q: Why Zinc(2+) in Lysis Buffer?
Wolfgang Schechinger
- Method for metaphase spreads: can you help?
g.coulton
- test do not read!
Sabine Hanelt
- Test do not read!
Sabine Hanelt
- test do not read!
Sabine Hanelt
- Test mal wieder please ignore
Sabine Hanelt
- Anybody getting unsolicited e-mail?
seppen at amc.uva.nl
- Keld Sorensen Apology
Margon Vandongen
- Automation of ABI fluorescent sequencing on a biomek robot
Hamish Scott
- Cheap Supplier of Carbenicillin ?
Ed MacLaurin
- Multiplex PCR
Sabine Hanelt
- **FREE Trial Pack of Middle School - College Essays**
Markus Reiser
- **FREE Trial Pack of Middle School - College Essays**
Markus Reiser
- Semliki vector pSFV1
William Moens
- anti CAT antibody wanted
Simon Twigger
- HELP - How to dissolve isobutylmethylxantine
Sinji Tauhata
- Make a $1,000,000 in 90 Days!
Greg Fitzgerald
- Make a $1,000,000 in 90 Days!
Greg Fitzgerald
- help with isoelectric focusing gels
Mr. J Hinshelwood
- (no subject)
Isidre Gibert
- seeking T7 promoter/polymerase for eukaryotic system
brett
- best mycoplasma detection kit?
David de Graaf
- Separating ssDNA from dsDNA (asymetric PCR) ???
Mr. J. Sasse
- Phosphotyrosine Abs-PY20 or 4G10?
Glenn Cantor
- oligo-dT regeneration
Edward Wang
- Test
Rob Hamilton
- Need plasmid map
Bill Zabaronick
- S35 cleaning
Peter Thoren
- Composition of M9 media.
sl3wx at cc.usu.edu
- ethidium bromide alternative?
Hector Cruz-Lopez
- Thermophilic DNA library?
Steve Bryson
- Composition of M9 media.
Brad Nicholson
- Need some different 6X Loading Buffers
Charles A Miller
- Qiagen's Direct mRNA kits
Pendragon
- Freezer Down - Is Taq still good?
COLINE at rulsfb.LeidenUniv.nl
- pUC repository?
DAB
- help: clean-up total RNA prep
Christopher M. Tebeau
- Antibodies to cGMP
doug feinstein
- Make a $1,000,000 in 90 Days!
Brad Thomas
- Restriction mapping software ??
Mark Darrin Osadjan
- Cosmid isolation
Jon Stewart
- Adding A's with taq
Jonathan Kurtis
- non-heat sterilization - correction
Jon Stewart
- Antibody purification questions
Frank Chen
- HELP - How to dissolve isobutylmethylxantine
Michael Clark
- Isolating buccal cells
Robyn Wallace
- plasmid multimers?
Hanbok Kim
- pK of lactobionic acid
Marc DIEU
- 6-His Ab/accessing archives
Dr. P.A. de Zoysa
- Li-Cor Sequencing apparatus
Mary P. Remington
- expression/secretion vectors
Dr Avri Davidoff
- Cloning vector(s) without ApaLI site(s)
RVDESHP0 at pwinet.upj.com
- Cheap carbenicillin
Simon Dawson
- apoptosis detection
Denise Lepley
- Air drier for DNA pellets?
Bob Kodrzycki
- BIOLUMINESCENCE LUCIFERASE BACKGROUND ACTIVITY
Fred De Leener
- Non-isotopic probe labeling kits
"Bridgette Berryhill ", BIO
- Air drier for DNA pellets?
pskaytes at INTNET.UPJ.COM
- Biomek 2000 workstation
Mary P. Remington
- Help!!! need a protein isolation protocol from leaves
J. Norrie
- RT-PCR/rTth RNA-PCR kit
Kamin Johnson
- Need Help extracting protein from 2D gels
Marc DIEU
- Desalting primer before electrospray mass spectrometry
Marc DIEU
- pMT21
Xin Guo
- Transcription run off
Neil Harris
- quantification of apoptosis
Abdelhamid Benazzouz
- RNA - CytoFluor2350 (Millipore)
SCHNEE at
- Ligation problems: large vector+small insert
Lena Polovnikova
- SexA I: digestion problems
Lena Polovnikova
- ABI's Taq FS
Jason Seto
- curing plasmid
Chi-Ju Wu
- seeking T7 promoter/polymerase for eukaryotic system
Marion Freistadt
- Tracking Dye
Eric Linton
- Removing exopolysaccharides and capsular polysaccharide
l14y at unb.ca
- SexA I: digestion problems
M. Alexeyev
- curing plasmid
M. Alexeyev
- How to dissolve EEDQ?
Steve Mcclue
- non-heat sterilization - suggestions?
LOGAN
- Subcloning pcr products
Dr I.M. Clark
- Subcloning pcr products
Albert Crescenzo
- Composition of M9 media.
M. Alexeyev
- centrifuge question
Nicole Anderson
- 6x Loading Buffers
Frank P. Johnston
- Subcloning pcr products
RVDESHP0 at pwinet.upj.com
- napthoquinone
Carine Michiels
- Aaarrgh -70 at +4C NIGHTMARE!!!!
John Dixon
- Need help extracting DNA from Epon embedded tissue
Roxanne Walder
- Uncut lambda DNA showing bands??
Knepper, Marc A
- Promoter sequence: help
Giorgio Spagnol
- Subcloning pcr products
Nikolai Chitaev
- Subcloning pcr products
Mayumi Yagi
- napthoquinone
mbro at seqnet.dl.ac.uk
- Size of BACTERIAL cDNA Library?
the End
- Need help extracting DNA from Epon embedded tissue
Dima Klenchin
- alpha-crystallin
Dana Anne Haley
- Aaarrgh -70 at +4C NIGHTMARE!!!!
Warren Gallin
- phage attack
Thomas Zander t3013
- Human Chromosome 15 Genomic library wanted
Carla Drebing
- no ligation of compatible fragments?
Michael Szardenings
- Baculovirus group?
Jennifer Lyon
- Automatic DNA extraction
Robert Roxby
- mini-preps: NOT kits.
Paul N Hengen
- gly or asn as spacers in proteins
Toby Lieber
- Seeking S. cerev. strain
Dan Baggott
- Pointer to FAQ list
Paul N Hengen
- PCR and Gels
Andy Kravetz
- Antibody (IgG) Purification
Mary Downing
- Non-radioactive RNase protection
musclephys at www-neuromus.ucsd.edu
- gly or asn as spacers in proteins
Ted Michelini
- Southern blotting from acrylamide gels?
JJAM
- Annealing and kinasing ssDNA oligos. Is it easy?
Sergey V. Orlov
- A protein synthesized at a bigger size in E. coli
Hirokazu KOBAYASHI
- pcr-primers
Jan Svensson
- Composition of M9 media.
John K. Troyer
- Sequencing PCR poduct w/ PRISM
Rob Fialho
- plasmid isolation
nc26 at wwa.com
- help with lipossome transfection!
Lygia da Veiga Pereira
- Removing exopolysaccharides and capsular polysaccharides
l14y at unb.ca
- Adsorbing antiserum of unwanted reactivity
jd89
- PLEASE READ - IMPORTANT change to METHODS-AND-REAGENTS mailing
list!!!
BIOSCI Administrator
- identifying TF seq.'s in a promoter
Ron Herzig
- mounting media?
Bryan Fox
- anybody an expert on Fiske & Subarrow assay?
Jon Nakamoto
- Internal vs external control
Jack Vanden Heuvel
- Transcription run off
Neil Harris
- Qiaquick Gel Kit
Andy Kravetz
- Southern blotting from acrylamide gels?
Sergey V. Orlov
- Removing polysaccharides
l14y at unb.ca
- Final Announcement - Research Fellowship
Cathie Stewart-Moore
- Qiagen extraction column
Makmal Mikrobiologi
- [Q] PCR gives extra band - heteroduplex formation?
Bernard Murray
- prokaryote-northern
beny spira
- Thio AMPc
J. GUERLOTTE
- fluore-dATP labelling mix for ALF auto sequencing
"Marianne Leverone ", BIO
- Cloninig problems!!!!!!!
Gareth Sullivan
- test-ignore
Bruce W. Ritchings
- Storage of Bacteria in microtitre trays - -80 or ???
Hamish Scott
- Recovering DNA from gels
jose
- test
smd8
- Size-Fractionation of DNA
colvin at ouvaxa.cats.ohiou.edu
- RNA and Protein from the same sample
S.K.Green
- [2]Re: Subcloning pcr products
To: Nikolai
- SSCP PAGE problems
Ayo Ola
- Invitro's WAX Mg Beads???
X
- RNA and Protein from the same sample
mbro at seqnet.dl.ac.uk
- Calculated and measured Mr
LOGAND
- pH of RPMI media for Hybridomas?
Frank Taddeo
- green fluorescent protein
Irina
- chicken embryonal eye cDNA bank ?
Burkhard Hassel
- Poly (U) Sepharose
Raquel Izumi
- why glycerol?
XZHENG at GAMMA.IS.TCU.EDU
- why glycerol?
Michael Gregory Abel, University of Tennessee
- Selection Problem in pUC18
Yeon Chul Kim
- Site directed mutagenesis kit
Chou Yat-Chen
- Fluore-dATP Labelling
AlanChan at compuserve.com
- ANOTHER TEST
R Daines
- Uncut lambda DNA showing bands??
Knepper, Marc A
- Design degenerate primers using Genefisher
Alex Chang
- Design degenerate primers using Genefisher
Soon Paik
- Anti HA Epitope Tag Antibodies
Tom Anderson
- RNA and Protein from the same sample
Madhu Singh
- Western blot of basic proteins
Robert Slany
- Extreme 5' end with RACE?
Friedemann Weber
- Problem Reference
Duncan Clark
- PCR fragment sequencing using ALF system
Oliver Politz
- E-mail addresses in France?
bbraun at ucla.edu
- 2-D gels with phosphoproteins
Patsy RENARD
- exonuclease source?
jane sandall
- Source for Replica plator pls.
Dr.Sailesh Surapureddi
- exonuclease source?
mbro at seqnet.dl.ac.uk
- CsCl-gradient pure whole phage M13, how?
Troels Wind
- Cycle sequencing
n4522701
- thermostable DNA dependent RNA polymerase
Juergen Brosius
- elution of RNA from acrylamide gels
Juergen Brosius
- Q:Li-Cor europ. adress?
COLINE at rulsfb.LeidenUniv.nl
- PCR of a GC Rich, Tandem Repeat Region: HELP!
Petros
- Cloninig problems!!!!!!!
brett
- Propidium iodide staining
Abdelhamid Benazzouz
- Bax and Fas Western detection
Abdelhamid Benazzouz
- Propidium iodide staining
Mayumi Yagi
- "Zap" entire cDNA library?
the End
- Help on nested deletion system
sa95078
- RNA and Protein from the same sample
straubek at VAX.CS.HSCSYR.EDU
- Help on nested deletion system
bio.tamu.edu
- Cycle sequencing
Bill Burnett
- Resuspension of very dry RNA?
workshop at dnatech.com
- Resuspension of very dry RNA?
workshop at dnatech.com
- Protein Halflife Technique
Aaron Pawlyk
- GST fusion protein
Ravi Mahalingam
- Help: Chicken IgY Handling & Stability
J S Abrams
- glucose
Dr.Sailesh Surapureddi
- Gentra Systems
Joan Boyce
- glucose
To: Nikolai
- Concealed
John Hachey
- (no subject)
musclephys at www-neuromus.ucsd.edu
- Looking for the Soruce for Replica plator pls.
Dr.Sailesh Surapureddi
- Using QuickTake for gel documentation
Dan Baggott
- Gene expression of adhesion molecules(CD2,ICAN1,B7,B70...etc)in
animal cell
Sang-Min Lim
- irreversible thiol(cysteine) proteinase inhibitor
l14y at unb.ca
- Blunt end cutter that cuts outside recognition sequence?
I'm not sure if there is such a thing, but does anyone know of any
Alistair Forrest
- Information
Maria Gastelum
- Acid-phenol
nian at pc-10.camden.usyd.edu.au
- Transient transfection
Caroline
- RNA and Protein from the same sample
Hirokazu KOBAYASHI
- Help: Smear on RT-PCR
Madhu Singh
- Enzymes differeces
Wachara Kasinreak
- salt conc. in in vitro transcription
kunheng luo
- PCR with 35S/32P-dATP
Nevil J Singh
- Invertase
R Daines
- AGAROSE MICROBEADS
d
- Strange Discrepancy Agarose/PAGE
Robert Greb
- subscribe
vanla dittapongpitch
- protease inhibitors
mbro at seqnet.dl.ac.uk
- electroporation of Pseudomonas
CDMENU at ccs1.cc.monash.edu.au
- Identifying a promoter: help!
Giorgio Spagnol
- glucose
ewingd at omrf
- Biologists and Kirkbrae Cricket Club
Mike Dalrymple
- Availability of HMT (4'-hydroxymethyl-4,5',8-trimethylpsoralen)
Juergen Sasse (Path)
- Rf: Cycle sequencing
LOGAND
- E. Coli Transformation
Stephen J. Palmieri
- Help: streaking of phosphopeptides on 16% gels
Patrick G. Swann
- Q: about immunoprecipitation
francisco silva
- BIOSYS
C.S.Wilding
- E. Coli Transformation
Mad Dan Eccles
- Q: about immunoprecipitation
Keld Sorensen
- Digitonin
Ian A. York
- DNA Binding to Filter Membranes
Duke Groebe
- Identifying a promoter: help!
bbraun at ucla.edu
- Map of cloning vector pBS(-)
Matthias Vogel
- E. Coli Transformation
Margon Vandongen
- BMB's Rapid DNA ligation kit
Harry Hahn
- Agrobacterium
Aleksander Sowa
- pMEP4 eukaryotic expression vector?
Namjin Chung
- alpha2-actinin antibody!
Nasrin Mesaeli
- E. Coli Transformation
Joaquim Culi
- Protein Labeling in E.coli
books at mcvax4.d48.lilly.com
- Transient transfection
Curt Ashendel
- Transfection of 3T3 adipocytes
Dezhong Yin
- Blunt End CIP Rxn
Barry Moore
- Blunt End Ligation Problems
Amadou Ba
- Blunt end ligation problems
Amadou Ba
- pH of RPMI media for Hybridomas?
To: Nikolai
- RNase protection kits?
bbraun at ucla.edu
- Is there a cutoff size for ethidium bromide DNA detection?
Daniel Kim
- Blunt end liagation problems
mbro at seqnet.dl.ac.uk
- DNA vs RNA antisense oligos
Dr. P.A. de Zoysa
- PE Taq FS-Multiple Peaks
williamssp at transcript
- help:hemoglobin content in ghosts
frederique GOUIN
- Thallium nitrate solns?
Joel Bunn
- binding studies
Clemens Suter-Crazzolara
- Q: looking for a source of rac and other rho family small G proteins
Wolfgang Schechinger
- Promoter Search:HELP!
Mari L. Shinohara
- PCR machines - recommendations?
Simon Twigger
- Antibodies to 6xHis fusions
Peter
- (none)
Thomas Hwang
- hybridoma fusion partners
Frederick Garbrecht
- HA-epitope
David L. Haviland, Ph.D.
- G418 and neomycin
Xin Guo
- Testing: Do not read
Sawyers Lab
- Testing: Do not read
ysong at ewald.mbi.ucla.edu
- Nerve Growth Factor ELISA (or RIA)
SCHNEE at
- Neutralizing Bioactivity
"wallace lauzon ", grad student
- unsollicited mail...
Wolfgang Schechinger
- luminometers
John M. Garrison
- Qiagen Lysis Buffer for RNeasy Kit
Tannis Beardmore
- Peptide Immunogens: ?Need for HPLC purification
Loren Joseph
- Assay for Tween-20
kmb at WH.BAYER.COM
- ****32-P PCR generated probe***
b.schonhoft
- Updated NIH grant templates for Mac Word and Excel available
Dennis Templeton
- HA-epitope
Bernard Murray
- 'A' addition during PCR
wakeman
- RNA from fixed cells?
Andreas Simm
- yeast colonyPCR
Kent Hill
- 'A' addition during PCR
Nikolai Chitaev
- G418 and neomycin
Nikolai Chitaev
- web sites
David Tak Yan Yu
- PEST-box predictions?
Paul Edward Kowalski
- gelshifts with supercoiled DNA?
David Haydon
- (none)
raghu at bcgoa.ernet.in
- 6in x 6 in Freezer box who sells them?
Shin Enomoto
- total RNA for Nothern from rabbit artery
Yoshiki Kawabe
- Thallium
Wolfgang Schechinger
- 32-P labelled probe
Zal Suldan
- 6in x 6 in Freezer box
Betty-anne Mcbey
- lambda gt10 or lambda zap
oser at uni-muenster.de
- PEG precipitation problem
Dr David A. Priestman
- test
Frank Chen
- Questions for cDNA library storage
Frank Chen
- Differential display vs RAP vs Delta RNA
Leonard A. Levin
- info needed on water conference
sbutcher at molbiol.ox.ac.uk
- web sites
Shaun D. Black
- SOE-PCR
Isabelle Ernest
- E.coli argU carrying plasmid
Dr. P.A. de Zoysa
- Making directed mutations in P1 bacteriophage
Tina T. Corkran
- ABI DNA synthesizer
natalie unruh
- Separation of two 55 kD proteins
Christopher B. Yohn
- Perkin-Elmer LS50
George W Chacko
- millipore ultrafree mc
anon at lamar.colostate.edu
- genomic library of Giardia lamblia
Francisco G da Nobrega ib - bio 7588
- Antisense for gene ablation in mice
mcatania at pathology.medsch.ucla.edu
- Antisense for gene ablation in mice
mcatania at pathology.medsch.ucla.edu
- G418 and neomycin
Sergey V. Orlov
- DNA quantification
lmjakt at hkucc.hku.hk
- Antisense gene knockout in mice
mcatania at pathology.medsch.ucla.edu
- Generic Lipofectamine
Enter Your Name Here
- New Method of Poly A+ Isolation
100145.3141 at compuserve.com
- MiniMACS
Mark D'Costa
- More PolyA+ questions/comments
Marc Lamphier
- GST fusions - please, help
Dima Klenchin
- Fe affinity chrom. & phospho-proteins?
Michael H. Bracey
- DNA Fragment Ends in PCR.
To: Nikolai
- plasmid blue or white colony
ali.ghadersohi at jcu.edu.au
- plasmid blue or white colony
ali.ghadersohi at jcu.edu.au
- plasmid blue or white colony
ali.ghadersohi at jcu.edu.au
- plasmid blue or white colony
ali.ghadersohi at jcu.edu.au
- plasmid blue or white colony
ali.ghadersohi at jcu.edu.au
- plasmid blue or white colony
ali.ghadersohi at jcu.edu.au
- plasmid blue or white colony
ali.ghadersohi at jcu.edu.au
- plasmid blue or white colony
ali.ghadersohi at jcu.edu.au
- plasmid blue or white colony
ali.ghadersohi at jcu.edu.au
- plasmid blue or white colony
ali.ghadersohi at jcu.edu.au
- plasmid blue or white colony
ali.ghadersohi at jcu.edu.au
- plasmid blue or white colony
ali.ghadersohi at jcu.edu.au
- plasmid blue or white colony
ali.ghadersohi at jcu.edu.au
- plasmid blue or white colony
ali.ghadersohi at jcu.edu.au
- plasmid blue or white colony
ali.ghadersohi at jcu.edu.au
- background in 2 hybrid screen
Ingrid Wolf
- background in 2 hybrid system
Ingrid Wolf
- ECL Optimisation
Seth Corey
- About acrylamide
Fung, Pik Yan Kitty
- cross-species hybridization
Karl Fischer
- Source of INA ?
pzhang
- Plasticizer leaching OFF microplates
MICROPLATE
- ASAP PLEASE -- Cloning PCR fragments
berns001 at coyote.csusm.edu
- cross-species hybridization
Chris McIntosh
- HA Epitope Tag Antibodies
Tom Anderson
- Lab Tech Position at Univ of Vemont
Rob Fialho
- (none)
fukami takaaki
- ECL Optimisation
Zhonglin Chai
- Looking for used Instrumentation
USTJD
- Looking for used Expedite DNA Synthesizers
USTJD
- plasmid blue or white colony
ali.ghadersohi at jcu.edu.au
- plasmid blue or white colony
ali.ghadersohi at jcu.edu.au
- lambda gt10 maxi-preps (non-recombinant)?
John Dixon
- RACE (Anchour) PCR
Simon Dawson
- Used biolab equipment wanted (East Coast)
Igor R Sagdeev
- MARCKS-Antibody
Andreas M. Stolpe
- B/T Blu
Mad Dan Eccles
- PCR product degradation
Sudha Paramale
- lexA antibody
Shin Enomoto
- TRizol
MUELLER
- Clontech 'Matchmaker' vector summary
bicfo at flinders.edu.au
- Qiagen Lysis Buffer for RNeasy Kit
QIAGEN
- ECL Optimisation
brett
- cross-species hybridization
Ian A. Boussy
- Imagene green and red
McCormick's Lab.
- ras
stefan.harjes at rz.ruhr-uni-bochum.de
- e.coli
Benke/Belmar Login
- beta-estradiol
lperal
- RACE - recommendations?
Peta C. Bonham-Smith
- Generic Lipofectamine
- histamine elisa
Robert Bateman
- pUC-BM20/21
Melanie Simpson
- *** FDA drug trials guidelines ***
b.schonhoft
- Biology Clip-Art
David J. Austin, Ph.D.
- Problems with Promega pGEM T-vector
Oivind Enger
- Problems with Promega pGEM T-vector
Oivind Enger
- Problems with Promega pGEM T-vector
Oivind Enger
- Problems with Promega pGEM T-vector
Oivind Enger
- Problems with Promega pGEM T-vector
Oivind Enger
- IMPORTANT: BIOSCI miniFAQ
BIOSCI Administrator
- unsubscribe
George Thomas
- Clontech Libraries and Matchmaker
cortines
- web sites
Armin Engels
- Microsatellite sequences
Henk Pennings
- *** FDA drug trials guidelines ***
Frederick Garbrecht
- E coli ghost prep
THORN at LAN.TJHSST.EDU
- PFGE APARATUS
simon day
- Problems with Promega pGEM T-vector
bio.tamu.edu
- PCR from plasma
Moniek de Maat
- Isopropanol precipitation
at hgu.mrc.ac.uk
- Isopropanol precipitation of DNA.
Donald Dunbar
- Problems with Promega pGEM T-vector
Simon Dawson
- Alternatives to Ethidium Bromide
G.W.Phillips
- Tetracycline inducible expression
Nigel Kenward
- SOS--PKC assay
Ying-Pi Lee
- Where to buy Protein A beads?
Mireille Moulard
- neurofibromin
stefan.harjes at rz.ruhr-uni-bochum.de
- Tropix pulls off a STING
Paul N Hengen
- Need a Gram-positive Expression Vector
Charles A Miller
- Enzyme Question (fwd)
Joshua Park
- help with silver stain
XZHENG at GAMMA.IS.TCU.EDU
- multiple sequences from Edman degrad
Bilbrey Robert
- GST fusion protein--pl help
Tay Hou Ngee Agnes
- (none)
fukami takaaki
- c-myc expression vector
Yasuyuki Kurihara
- E-mail in Switzerland
Caroline
- primer staining mystery
Manfred Schuermann
- Cary 1E uv/vis spec and OS/2
Manoj Ramjee
- Nunc tissue culture inserts
G.P.
- Southern blotting from acrylamide gel ?
Toshiharu Ishizuka
- Alternative to Ethidium Bromide
mbro at seqnet.dl.ac.uk
- dna in cells
Benke/Belmar Login
- GeneView
Veronika Gaal
- RNA Isolation from Whole Blood
Barry Vipond
- Replica Plating of Yeast?
Mike Curtin
- Clonetech Quantun Leap deletion kit.
Phillip Lord
- Fold Induction with lacUV5 promoter
taylor
- Test post
Jim_LeMay at fws.gov
- Extracting clean DNA from large flies
P. Emery
- RNA Isolation from Whole Blood
Anne Spurkland
- Molecular Vision, a new Web Journal
Jeffrey H. Boatright
- Help with UV Shadowing requested
Stephen Marcus Lane
- How disrupt fungal cells
Michel Traris
- AFLP marker
EVELYN MOELLER
- Frequently Asked Questions (FAQ) list Pointer
Paul N Hengen
- M13 as probe for mammalian minisatellites
W.J. Hopkins
- Overexpression of a protien (fwd)
Natalie Lu
- Isopropanol precipitation of DNA.
Dr.Sailesh Surapureddi
- FISH manuals and courses
Andreas Kalmes
- FISH manuals and courses
Andreas Kalmes
- FISH manuals and courses
Andreas Kalmes
- beta shield sources
Rob Jordan
- Sequence of 5' -region of human mas-oncogene
Vladimir Yu. Kuryshev
- Tetracycline inducible expression
Seth Corey
- Tetracycline inducible expression
Seth Corey
- Antibiotic Resistance Cassette Update
Paul N Hengen
- h. pylori antigen
LEE
- Subcloning of PCR products.
yeast at imtech.ernet.in
- Q: Double digestion of adjacent restriction sites ?
stronger
- Perfect-match PCR enhancer
Carolyn Calnan
- LOOKING FOR USED EXPEDITE DNA SYNTHESIZER
USTJD
- Tet inducible expression
Graham Atherton
- DNA analysis software choice
mbwen at ccvax
- DNA analysis software
Graham Atherton
- MUF-a.a. Source???
Christian Belanger
- Detergent less foamy than SDS.
Christian Belanger
- test mail 1
Ruud Ross
- microwave and DNA
ynakao at kean.ucs.mun.ca
- Origin of Transfer cassetes
Charles A Miller
- Waters LACE IEEE-Card sought
Christoph Gartmann
- Re-using Wizard Minipreps
JG. Head
- FISH manuals etc.
Wilfried Rossoll
- Pharmacia Recomb. phage Ab kit (ScFv)
Deb Britt
- Origin of Transfer cassetes
Paul N Hengen
- pRSV Beta-gal
Jeanmarie Donovan
- suitable protease negative, lacIq E.coli
sl3wx at cc.usu.edu
- Arabidopsis transformation
Rafael Maldonado
- DYNAL Biotech
susan larchy
- Pharmacia Recomb. phage Ab kit (ScFv)
RVDESHP0 at pwinet.upj.com
- Need some help with the P1 information
Tina T. Corkran
- Replica Plating of Yeast?
Rafael Maldonado
- Beyond FAQ - Seeking further advice on primer design
Doug Eernisse
- Transfection of VERO cells??? Anyone???
Alistair Forrest
- optimization for generating recombinant Adenoviruses
Edgar Schreiber
- p53 clone - p53.doc [01/01]
Theo Dopheide
- p53 clone
Theo Dopheide
- Lambda-HindIII ladder
Thomas Chuang
- RACE
Yasuhito Abe
- TRANSFECTION of Human Monocytes
Dirk Jochmans
- DNA extraction from ruminant placenta-help please!
Mr M.R. Owen
- Overexpression of a protein
cwilliams
- is quantitative blot possible ?
Stefan Bäckström
- Bacterial DNA
Surasakdi Wongratanacheewin
- test post
Christian Belanger
- test post
Christian Belanger
- Dynal
workshop at dnatech.com
- DNA Software
workshop at dnatech.com
- electroporation of hepatocytes ?
Maria Thuveson
- Lambda-HindIII ladder
M. Alexeyev
- Lambda-HindIII ladder
Margon Vandongen
- Extracting clean DNA from large flies
Steven Russell Genetics
- beta-gal staining of BHK21 cells
M. Alexeyev
- Where is hflA E.coli tet-sensitive strain?
Mark Walberg
- multiple sequences from Edman degrad
Shaun D. Black
- Need: DNA mini-prep for hard-shell beetles
tom bigham
- DNA contamination in RT-PCR
Isidre Gibert
- DNA in RT PCR Rx
workshop at dnatech.com
- CTAB method
Michael Cooley
- DNA contamination inRT-PCR
Andrei Popov
- RNA/protein discussion group
Keith Hoek -BIOCHEM
- TRIVALENT ARSENOXIDES: Spectral data ???
Peter Happersberger [Chemie]
- the function of -70 for X-ray film
lIQIN WANG
- pH
Sudha Paramale
- Removal of Melanin from DNA/RNA
Madhu Singh
- Wanted: Mouse B-cell cDNA library
Shane Albright
- the function of -70 for X-ray film
Shane Albright
- Best Method for RNA/DNA Prep.
NEVIL J SINGH
- B.thuringiensis protein expression in E.coli
KIMMO KOIVU MMKAT_OPISK
- fungal cultures
Annick Wilmotte
- the function of -70 for X-ray film
Nikolai Chitaev
- THP-1 transfection?
Hamid_Khoja at cc.chiron.com
- MAGNETIC POROUS GLASS
DNABILL
- Tritiated-thymidine labelling for replication
David Lukac
- Know a stain FOR agarose (in histo wax sections)
David Parsons
- the function of -70... (correction)
Nikolai Chitaev
- THP-1 transfection?
brett
- Mutagenesis Blues
Karl Fischer
- Determining quality of polyA+ RNA
Marc Lamphier
- AP-PCR/RAPD directly from sigle colonies??
Martin Laurell
- Tritiated-thymidine labelling for replication
Nikolai Chitaev
- Mutagenesis Blues
Yasha Hartberg
- cyclone synthesizer
doug feinstein
- In vivo footprinting and RNA-oligo capping
Heng Lu
- Mutagenesis Blues
Yasha Hartberg
- PCR-Quantitative and NO GELLS
gwade at physiology.uwo.ca
- DNA extraction from blood
Kizanne Davies
- competent cell preparation
lees at SASK.USASK.CA
- MHC(I) locus A mAbs
Anatoly Sharipo
- Inducing random frameshifts with PCR??
Michael Hamilton
- Cre expressing mice
Dr. M Raabe
- Ligation buffer constituents
M.C.Behrendt
- Problems with PCR cloning
AG Melkonian
- DNA extraction from single paraffin sections
J.A. Randerson
- Overexpression of a protien (fwd)
Geoff Briggs Dr.
- Chick Neuron Cultures
Mr D.J.A. Wilson
- HSV-tk
Nobuyuki Enomoto
- Fluorescent covaspheres
Mr D.J.A. Wilson
- Yeat miniprep lits?
Hamid_Khoja at cc.chiron.com
- Streptococcal Mini-Prep.
M.C. Fontaine
- HELP-33P LABEL IN GELSHIFT EXPERIMENTS
Eric Hanson
- RXR plasmids
Jack Vanden Heuvel
- Determining quality of polyA+ RNA
CK Wen
- assistance needed
neuro
- Info needed on Gibco comp cell protocol
Lex
- western blots
Denise Lepley
- Isolating areas flanking a gene
William Driscoll
- protein expression
ynakao at kean.ucs.mun.ca
- c myc antibody
SHERMAN LAB
- Storage/stability of DNA
Bruce Zuraw
- (none)
Normando Iznaga
- Isolating areas flanking a gene
Brad Nicholson
- Chaotropic agens?
AztecLee
- U373 cell transfection?
Hamid_Khoja at cc.chiron.com
- Yeast plasmid nminipre Kits?
Hamid_Khoja at cc.chiron.com
- Slot blot of PCR rxns?
Lauryl Maiya J Nutter
- Cutting M13 dsDNA
Jo Caine
- Postdoc position in St. Louis
Wayne M. Barnes, Ph.D.
- Position in Dallas
Mark Walberg
- Isolating areas flanking a gene
wally at bio.tamu.edu
- where to go for custom in situ hybridization for mRNA?
Kenneth Y. Ilio
- Chick Neuron Cultures
straubek at VAX.CS.HSCSYR.EDU
- NIH-grant templates for Mac now on major servers
BIOSCI Administrator
- Re-use of magnetic beads in Promega's PolyA selection system
Lely Chow
- Q: GS system & BHK cell
Kernee H.Chang
- primer extension with A.L.F.
Oliver Politz
- western blots
Dima Klenchin
- where to go for custom in situ hybridization for mRNA?
Jukka Luoma
- hplc of plasmids/small DNA fragments?
Stephan Witte
- postdoc position
Yair M. Heimer
- Anti-Immigration Law Status/Seminar
Brian B. Jiang
- help!!!Where can I buy HA/protease or P-elastase?
Zhengyang Wu
- Need killer gene
jim hartley
- protein expression
ynakao at kean.ucs.mun.ca
- Plasmid Drawing Programs for PCs?
Rob Robson
- help!!!Where can I buy HA/protease or P-elastase?
Zhengyang Wu
- Rf: PAGE: Run times and voltages
LOGAND
- Q: best way to store southerns
Karl Worm
- pBLCATPA
dr. P . Vreken
- Transfection of Primary Rat Hepatocytes
Martijn Groenink
- Gluthation S-transferase inhibitor
reichhart at astorg.u-strasbg.fr
- Any differences between mitosis in transformed and untransformed cells?
Dietmar Baas
- Frankfurt, Brussel, Paris-->Seoul via Singapore
Jae-Seong Lee
- test post #3
Christian Belanger
- protein extraction from SDS-Page
lw75
- DAPI question
Sarah Tamara Braun
- protein extraction from SDS-Page
Frederick Garbrecht
- Q: best way to store southerns
David L. Haviland, Ph.D.
- test
Hamid_Khoja at cc.chiron.com
- U373 cell transfection?
Hamid_Khoja at cc.chiron.com
- Yest plasmid miniprep kits?
Hamid_Khoja at cc.chiron.com
- Rf: PAGE: Run times and voltages
Ian A. York
- 6XHis bacterial expression vector
Dr. Prasun K. Datta
- High Resolution mini-gels?
Petros
- Overexpression of protein
Clemens Suter-Crazzolara
- Need help re: dam methylase sensitive RE
Randall Dreger
- RNA STABILITY
stefan.harjes at rz.ruhr-uni-bochum.de
- Any opinions or recipes for Taurine in sequencing buffer
Dr. M.R.Barnes
- Budget nonradioactive methods/kits
Michael R. Frank
- Designing PCR primers
Taunja Lee Palmer-Stone
- competent cell preparation
Ken Howe
- Storage/stability of DNA
Andrei Popov
- RT-PCR without RNA extraction...
Fang-Yao Hou
- Agarose gel electroforesis buffer
Yap Yun Kiam
- video records of gels
David Tak Yan Yu
Last message date:
Tue Oct 31 23:11:17 EST 1995
Archived on: Sun Mar 6 20:47:35 EST 2005
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