Antibody for His-Tag

Tomas Drgon tomasd at bdg10.niddk.nih.gov
Fri Sep 1 08:36:56 EST 1995


In article <425bn3$koa at netnews.upenn.edu>, lally at toxin.dental.upenn.edu
(Ned Lally) wrote:

> In article <tomasd-0609561722140001 at fp5-9n.domain>,
tomasd at bdg10.niddk.nih.gov (Tomas Drgon) writes:
> |> 
> |> They say it "sees" Met-Arg-Gly-Ser-(Hisx6). So it might see also
(Hisx6) alone.
> |> Did you try it out?
> 
> Didn't need to do it, the Ni+-NTA-PO4 worked fine for me.
> 
> Six tandemly expressed His are no doubt very immunogenic and since an
antibody 
> combining site is 6-8 aa there is no doubt that some of the antibodies
recognize 
> the His-tail. But how much, I don't have any idea, I didn't make the
antibody. 
> Qiagen recommends the antibody on vectors expressing
Met-Arg-Gly-Ser-(Hisx6) you can bet
> that that a significant part of that titer is tied up in some part of
Met-Arg-Gly-Ser.
> What do you think??    
> 
> Bye,
> Ned
>     
>     
You're probably right. I have never used it, I was just guessing.

Tomas

-- 
I speak for nobody but myself and my pet frog.



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