HELP: PCR Subtractive Hybridisation/reliable method wanted.
g.coulton at cxwms.ac.uk
Fri Sep 1 08:28:50 EST 1995
I am in the planning stages of a project which with depend to a large
degree on the production of enriched cDNA libraries. I have screened
the literature and come up with any number of variations on the theme. I
need to know which to include in the strategy and why.
So can you help?
My starting material will be whole skeletal muscle. Yes, I know that it
is multicellular and all that this brings but I am stuck with this
situation at the moment.
Can you either point me in the direction of a particular paper or can
you send me your protocol?
Thanks in advance.
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