Faint PCR bands-Help!

Andrei Popov ANDREI.POPOV at bbsrc.ac.uk
Mon Sep 4 08:38:16 EST 1995


>From: edahl at moose.uvm.edu (Eric N. Dahl)
>Subject: Faint PCR Bands-Help!
>Date: 4 Sep 1995 02:07:52 GMT
>
>I am trying to amplify a 1 kb fragment and have been getting faint
>bands. My PCR conditions are pretty standard and I've tried playing with
        ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
>the Mg conc. and primer concentration without much success.  I also tried
>reamping and got slightly better bands but still not bright enough.
>
>I'd appreciate any suggestions! 

Dear Eric,

please be more specific, otherwise your posting
and most of our suggestions are most likely to be 
just a waste of time.

Best regards

Andrei Popov




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