Literature needed / Biotechniques 13

[Richard Rohan]

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Rich Rohan

Christoph <kruell at rz.uni-duesseldorf.de> wrote:

>Hello everybody!

>I need some information about the following literature which is 
>unavailable for me:

>Stemmer and Morris: "Enzymatic inverse PCR: a restriction site independent, 
>single-fragment method for high-efficiency, site-directed mutagenesis"
>Biotechniques 13: 214-220 (1992)

>So far I only have the abstract. Could someone be so kind and answer 
>my questions about that literature. Which specific restriction 
>enzyme did they use? What is the sequence of the primers they used?

>Thank you for the effort!

>Christoph

>christoph.kruell at uni-duesseldorf.de