Proving (subcloned) A549 cells are truly A549 cells

Simon Twigger simont at post.its.mcw.edu
Tue Sep 5 11:55:37 EST 1995


I believe that I have subcloned an A549 cell line that has some
different characteristics (increased cell cycle and decreased 
adherence, for example) from the newly purchased ATCC A549 cells.

How do I prove (for publication purposes) that the subcloned cells
are (were?) truly A549 cells?

Southern blotting/RFLP analysis has been suggested - would this be
enough?

Any ideas/approaches or references of others who have done similar
tasks would be greatly appreciated

Thanks,


Jennifer Rasmussen
Dept Biochemistry
Med. College of Wisconsin



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