problem with expression using baculoviruses

smorgan at fs1.ho.man.ac.uk
Tue Sep 5 10:12:13 EST 1995


I have been unable to express HLA-DQ glycoproteins using the 
baculovirus/insect cell system (detection by Western blotting).
I inserted the genes in pAcHLT-C and cotransfected with BaculoGold into 
Sf9 and High 5 cells but with no luck. 

Does anyone have any explanations or advice as to why I have been 
unsuccessful?

Look forward to some help.

Shethah Morgan.  




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