protein expression systems

Darren Fast fast at
Tue Sep 5 18:21:27 EST 1995

In article <42huuk$8ba at>, m-brazil at (Melanie
Brazil) wrote:

> we want to express the serum complement protein C5 in an expression system.
> The cDNA is 5kb long, and the protein is very large - 200kDa.
> Would anyone recommend either Bacculovirus or Picchia Pastoris as more
> suitable for a large protein? We have tried Schneider Cells but have been
> unsuccessful.
> Are there any others systems that we have missed?

We have sucessfully expressed a 250 kDa protein (apoB48) in baculovirus. 
So it can be done.


Darren Fast
fast at
Dept of Biochemistry
University of Wisconsin-Madison

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