protein expression systems

[Darren Fast]

In article <42huuk$8ba at mserv1.dl.ac.uk>, m-brazil at nimr.mrc.ac.uk (Melanie
Brazil) wrote:

> we want to express the serum complement protein C5 in an expression system.
> The cDNA is 5kb long, and the protein is very large - 200kDa.
> 
> Would anyone recommend either Bacculovirus or Picchia Pastoris as more
> suitable for a large protein? We have tried Schneider Cells but have been
> unsuccessful.
> 
> Are there any others systems that we have missed?


We have sucessfully expressed a 250 kDa protein (apoB48) in baculovirus. 
So it can be done.

Darren

-- 
Darren Fast
fast at biochem.wisc.edu
Dept of Biochemistry
University of Wisconsin-Madison
(608)-262-4705