Help on site-directed mut
harold.drabkin at channel1.com
Wed Sep 6 00:27:00 EST 1995
Subject: Help on site-directed mutagenesis kit
E>Could someone advise me on what is the best kit to buy
E>to perform site-directed mutagenesis on a long cDNA.
E>What I mean buy best is easy to handle, fast and good
E>results. How about Chameleon from Statagene? Thanks in
I use the Amersham kit routinely. It's a bit on the expensive side, but
the results are very good.
One word of caution, however: Unless you wish to sequence verify the
entire gene, I would consider subcloning the smallest fragment needed to
mutagenize and sequence verify, then clone it back into the full
* OLXWin 1.00 * 141
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