HELP, PCR OF GC RICH TEMPLATE.
Dlevitzke at medicine.newcastle.edu.au
Tue Sep 5 18:19:01 EST 1995
I am currently working with an ABI 377 Automated DNA Sequencer. Part of
this machines applications lie in Genescan, ie the sizing and
quantitating of samples. I am currently working on a project to size
the repeat region of Fragile-X syndrome. Because it consists of a GCC
repeat region it is notoriously hard to PCR, if anyone has any
experiance/advice for the amplification of GC rich templates I would
greatlty appreciate the input.
Thanks heaps. Dale Levitzke.
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