SP6 Polymerase

David Figueroa figueroa at DNAX.ORG
Thu Sep 7 19:22:48 EST 1995

I have also found these polymerases to give me different yields. In my
transcription reactions,I use 3 times higher concentration of SP6 than T7. I
incubate the rxns at 37C for about 2.5 hrs and usually get equal products.

I'd be very interested in any references where efficiencies of different
polymerases have been compared.

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