SP6 Polymerase

David Figueroa figueroa at DNAX.ORG
Thu Sep 7 19:22:48 EST 1995


Walter:
I have also found these polymerases to give me different yields. In my
transcription reactions,I use 3 times higher concentration of SP6 than T7. I
incubate the rxns at 37C for about 2.5 hrs and usually get equal products.

I'd be very interested in any references where efficiencies of different
polymerases have been compared.




More information about the Methods mailing list