help: primer restriction sites

Kimberly Walker PhD kwalker at
Thu Sep 7 16:34:22 EST 1995

On 31 Aug 1995, Igor R Sagdeev wrote:

> The table for enzyme efficiencies near the end of DNA fragment
> is great! However, the list of enzymes in the tables is very
> limited. Is it safe to assume that for other enzymes (I'm interested 
> in NciI and HhaI) the efficiencies are 90% or higher when the
> restriction site is flanked by 3 or more bp?
Everyone i know who has done this sort of thing thinks you need 5 or more 
BP's on the end of a primer to make SURE the RE actually cuts.  

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