help: primer restriction sites
Kimberly Walker PhD
kwalker at umabnet.ab.umd.edu
Thu Sep 7 16:34:22 EST 1995
On 31 Aug 1995, Igor R Sagdeev wrote:
> The table for enzyme efficiencies near the end of DNA fragment
> is great! However, the list of enzymes in the tables is very
> limited. Is it safe to assume that for other enzymes (I'm interested
> in NciI and HhaI) the efficiencies are 90% or higher when the
> restriction site is flanked by 3 or more bp?
Everyone i know who has done this sort of thing thinks you need 5 or more
BP's on the end of a primer to make SURE the RE actually cuts.
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