pGL2 insert fragment sizes

[zhangxia at post.queensu.ca]

In article <patterson.1160794136B at oitnews.harvard.edu>, patterson at cvlab.harvard.edu (Cam Patterson) says:
>
>I have  cloned 15.5 kb into pGL2 basic. Wasn't that hard once I stopped 
>trying to gel purify the fragment and just did shotgun cloning
>followed by colony lifts.
>
>cam

I am just curious about what's the problem when you tried to gel purify
the fragment and cloning since I am having difficulty to do the cloning
with the pGL2 basic.

Thanks

Xia

zhangxia at post.queensu.ca