help: primer restriction sites

[Joanne D. Andreadis]

On 31 Aug 1995, Igor R Sagdeev wrote:

> The table for enzyme efficiencies near the end of DNA fragment
> is great! However, the list of enzymes in the tables is very
> limited. Is it safe to assume that for other enzymes (I'm interested 
> in NciI and HhaI) the efficiencies are 90% or higher when the
> restriction site is flanked by 3 or more bp?
> 

I would try seven or eight flanking bps beyond the recognition site if 
you do not know anything about the restriction enzymes. Might as well 
give the enzyme a nice comfortable site to rest on.