Rnase Protection Assays

[Francois Nantel]

In article <1995Sep5.161710.9596 at alder.cc.kcl.ac.uk> ,
rcha244 at bay.cc.kcl.ac.uk writes:
>Can anybody out there tell me whether it is absolutely necessary to gel 
>purify your labelled RNA probe prior to hybridisation with the target 
>RNA. If not it would make the technique much more user friendly. 
>Thanking you in anticipation.
>
I've never done and never needed to. I usually either precipitate the
probe or purify it on a spun column.

Francois