Rnase Protection Assays

Francois Nantel Nantel at titus.u-strasbg.fr
Fri Sep 8 07:46:40 EST 1995

In article <1995Sep5.161710.9596 at alder.cc.kcl.ac.uk> ,
rcha244 at bay.cc.kcl.ac.uk writes:
>Can anybody out there tell me whether it is absolutely necessary to gel 
>purify your labelled RNA probe prior to hybridisation with the target 
>RNA. If not it would make the technique much more user friendly. 
>Thanking you in anticipation.
I've never done and never needed to. I usually either precipitate the
probe or purify it on a spun column.


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