Faint PCR Bands-Help!

Dale Levitzke Dlevitzke at medicine.newcastle.edu.au
Sun Sep 10 18:25:15 EST 1995


TRY DECREASING YOUR ANNEALING TEMPERATURE TO DECREASE THE SPECIFICITY OF 
YOUR PRIMERS.  ALSO 5-10% DMSO OR GLYCEROL OR COMBINATIONS OF BOTH MAY 
HELP TO INCREASE THE OVERALL ROBUSTNESS OF YOUR REACTION.





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