Protein standards for DENATURING (9M urea) IEF
Stephen P. Driska PhD
driska at astro.ocis.temple.edu
Mon Sep 11 10:21:41 EST 1995
[ Article crossposted from bionet.molbio.proteins ]
[ Author was Stephen P. Driska PhD ]
[ Posted on 11 Sep 1995 15:18:19 GMT ]
Many companies sell protein standards for isoelectric focusing
(IEF), but it seems that nearly all of the products are intended for
NON-DENATURING IEF of native proteins. I'm looking for a standard
mixture for IEF under denaturing conditions, e.g. 9M urea, Nonidet P-40,
The only product I have seen that seems like it would be
appropriate is the Sigma kit for 2-Dimensional electrophoresis. This
kit only has 4 proteins, though, so it might not be complete enough for
what I want to do.
There are lots of mixtures for 1D-SDS electrophoresis, but I
wouldn't necessarily expect these standards to have nice banding
patterns in IEF gels.
My question is, can any one tell me where I might buy a
standard mixture for denaturing IEF, or perhaps recommend a selection of
known proteins which could provide nice bands in 9 M urea ?
Thanks very much for your help,
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