Universal bases in PCR primers

Mike Dalrymple dalrymple at pplros.demon.co.uk
Mon Sep 11 02:45:39 EST 1995

>Dear Ron, why do you want to use them? are they cheaper than inosine?  Mike
>m.witty at ic.ac.uk

My understanding of inosine is that it prefers base pairing to A,C and T
G and therefore is not truly universal. 3-nitropyrolle (?is that right?)
is a better "universal" base.

Mike D.

More information about the Methods mailing list