SP6 RNA polymerase -- HELP

Warren Gallin wgallin at gpu.srv.ualberta.ca
Tue Sep 12 10:47:10 EST 1995

In Article <433rag$q5p at griffin.ccc.nottingham.ac.uk>, Nigel Kenward
<mbxnjk at vax.nott.ac.uk> wrote:
>Hi There,
>Unfortunately SP6 NEVER works as well as T7 or T3 !
>I guess it's something to do with the fact that the T7 and T3 are viral 
>in origin and have to work better in-vitro to do-the business for the 
>virus. SP6 is bacterial and therefore probably doesn't need to be as 

In fact, all three are isolated bacteriophage polymerases.  SP6 comes from
Salmonella infected with the SP6 phage, hence the name.  Also, I don't think
you will get universal agreement about SP6 being substantially poorer than
T7, and certainly not T3.

Warren Gallin,
Department of Biological Sciences, University of Alberta
wgallin at gpu.srv.ualberta.ca

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