Rnase Protection Assays
David L. Haviland, Ph.D.
HAVILAND at KIDS.WUSTL.EDU
Mon Sep 11 12:17:26 EST 1995
In <1995Sep5.161710.9596 at alder.cc.kcl.ac.uk> rcha244 at bay.cc.kcl.ac.uk writes:
> Can anybody out there tell me whether it is absolutely necessary to gel
> purify your labelled RNA probe prior to hybridisation with the target
> RNA. If not it would make the technique much more user friendly.
> Thanking you in anticipation.
Purify the probe, yes, through a gel? That's up to you. Personally, I
used the G25 spin columns from BMB that were sold as being RNAse free.
These worked very well for me when I was doing RNAse protection assays.
Hope this hels,
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