Rnase Protection Assays

[David L. Haviland, Ph.D.]

In <1995Sep5.161710.9596 at alder.cc.kcl.ac.uk> rcha244 at bay.cc.kcl.ac.uk writes:

> Can anybody out there tell me whether it is absolutely necessary to gel 
> purify your labelled RNA probe prior to hybridisation with the target 
> RNA. If not it would make the technique much more user friendly. 
> Thanking you in anticipation.


Greetings Paul:

Purify the probe, yes, through a gel?  That's up to you.  Personally, I 
used the G25 spin columns from BMB that were sold as being RNAse free.  
These worked very well for me when I was doing RNAse protection assays.

Hope this hels,
David