direct PCR amplification using library

AShaw5 ashaw5 at
Tue Sep 12 23:51:02 EST 1995

I agree completely.  I have never been successful PCRing from a library. 
And it is usually no problem by RT-PCR.  I think that it is due to the
HUGE amount of DNA that is contributed by the vector.  
Andrey Shaw

More information about the Methods mailing list