Glass beads/ acid-washed

Paul N Hengen pnh at fcsparc6.ncifcrf.gov
Tue Sep 5 12:42:32 EST 1995


In article <1995Sep5.175726.1 at hkucc.hku.hk> vldnaseq at hkucc.hku.hk writes:

| I have bought some glass beads for lysis of the bacterial cells with a bead
| beater.  Before using these, I know that the glass beads should be
| acid-treated.  However, I do not know how to perform the acid-washed, and the
| following maintainence of these beads after use.  Would anyone share your
| experience?  Thank you.  Shannon

It's not worth the trouble! Buy them prewashed and ready for microorganism
disruption -> Sigma G-4649

But if you want to reuse them, soak in 0.1 M HCl, rinse several times with
dH20, spread them out on a cookie sheet and bake in the oven for a few hours
to dry and sterilize.

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