Precipitate in DIG Hybridization Solution?
h.woo at sct.gu.edu.au
Wed Sep 13 01:30:37 EST 1995
I have noticed presence of precipitate, at the end of pre-hybridization
and hybridization, in the hybridization solution recommended for
DIG-labelled probes by Boehringer Mannheim. The composition of the
solution is as follows:
1% Blocking reagent
Do other DIG users have similar observation? Can this cause failure of
I did the hybridization at 51C inside a Hybaid roller-bottle oven.
Heating the solution containing the precipitate in boiling water bath for
10 min did not dissolve the precipitate.
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