ligase reaction/how long ?

Tomas Drgon tomasd at
Wed Sep 13 09:58:41 EST 1995

In article <436mr1$ibj at>, Clemens Suter-Crazzolara
<un691cs at> wrote:

> hello,
> i would be happy to hear what average time you use for
> t4 ligase reactions of sticky ends. some companies suggest
> that 1 hour is enough at RT for maximum ligation,
> some colleagues even suggested 5 minutes (!).
> Did anybody ever test this out ?
> clemens

In my hands 30' at RT worked ok. I used Pharmacia Ready-To-Go premade
ligase/buffer mixes.


I speak for nobody but myself and my pet frog.

More information about the Methods mailing list