ligase reaction/how long ?

Tomas Drgon tomasd at bdg10.niddk.nih.gov
Wed Sep 13 09:58:41 EST 1995


In article <436mr1$ibj at mserv1.dl.ac.uk>, Clemens Suter-Crazzolara
<un691cs at genius.embnet.dkfz-heidelberg.de> wrote:

> hello,
> i would be happy to hear what average time you use for
> t4 ligase reactions of sticky ends. some companies suggest
> that 1 hour is enough at RT for maximum ligation,
> some colleagues even suggested 5 minutes (!).
> Did anybody ever test this out ?
> 
> clemens

In my hands 30' at RT worked ok. I used Pharmacia Ready-To-Go premade
ligase/buffer mixes.

Tomas

-- 
I speak for nobody but myself and my pet frog.



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