? about mtDNA isolation

Juliewoods juliewoods at aol.com
Wed Sep 13 21:59:52 EST 1995


Perhaps someone reading this has experience with CsCl fractionation of
mtDNA?  When the mtDNA is digested with restriction enzymes, end labelled
with P32 and run on a 1% vertical agarose gel, dried and exposed to film
-- what causes the faint radioactive smearing between the bands?  It has
been suggested that this indicates the presence of linear genomic
fragments in the prep!  Does this sound likely?  Thanks for any thoughts
about this -- julie.  



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