SSCP gel smiling problem

Greg Niemi niemiga at u.washington.edu
Wed Sep 13 17:15:35 EST 1995


Does anyone have any advice on how to reduce smiling in SSCP gels? I am 
using a BRL sequencing gel box and have very significant smiling of my 
bands in the bottom half to third of my gel. It happens if I run the 
gels at room temp or at 39 degrees C. I am running out a digested 32P 
labelled PCR product on the gel.

Also I am wondering if people have any experience comparing isotopic 
SSCP vs. non-isotopic SSCP. I would prefer to use a non-isotopic method  
such as silver stain if possible.

Thanks,

Greg





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