SSCP gel smiling problem
niemiga at u.washington.edu
Wed Sep 13 17:15:35 EST 1995
Does anyone have any advice on how to reduce smiling in SSCP gels? I am
using a BRL sequencing gel box and have very significant smiling of my
bands in the bottom half to third of my gel. It happens if I run the
gels at room temp or at 39 degrees C. I am running out a digested 32P
labelled PCR product on the gel.
Also I am wondering if people have any experience comparing isotopic
SSCP vs. non-isotopic SSCP. I would prefer to use a non-isotopic method
such as silver stain if possible.
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