EtBr filter for CCD camera?
hemant at hoh.mbl.edu
Wed Sep 13 15:08:22 EST 1995
In article writes:
>From: Pavel Sova <ps44 at columbia.edu>
>Subject: EtBr filter for CCD camera?
>we have a neat setup of CCD camera connected to the computer and are
>taking pictures of anything short of EtBr stained DNA on agarose gels.
>When I tried to do this, camera took very high bacground of UV bulbs,
>even showing some numbers written on bulbs. I wasn't able to block this
>background by orange filter, which we routinely use for photography of
>EtBr stained DNA on agarose gels (on Polaroid films). Neither UV blocking
>filter did any difference.
>I think that some of the light emitted from UV source is actually of visible
>blue color, which goes through UV blocking filter and also through orange
>filter. Do you have anybody suggestion what filter shoud we use (and it's
A simple solution would be to irradiate the gel from the side like so:
This would eliminate direct UV from getting to the camera.
The standard screw-on orange filter would suffice.
Hope this helps. Good luck.
Dr Hemant Chikarmane, Marine Biological Laboratory, Woods Hole, MA 02543 USA
email: hemant at mbl.edu voice: (508) 289-7245
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