resuspend DNA

[Tim Barnett]

First, make sure you don't dry the DNA pellet too well as this makes it
very hard to re-dissolve.  Then add the required amount of TE buffer (pH
8.0) and redissolve by pipetting (for small fragments where shearing is not
a problem) or by placing in a 60 degree water bath for about 2 hrs (for
larger fragments).

Tim

In article <chenz-1209952203250001 at 131.215.5.168>,
chenz at starbase1.caltech.edu (zhoufeng chen) wrote:

> add water or TE.     In article <9509121249.AA49630 at server.indo.net.id>,
> darmonot at SERVER.INDO.NET.ID (Darmono Taniwiryono) wrote:
> 
> > What is the best way to resuspend dried DNA pellet ??  Thanks.
> 
> -- 
> zhoufeng chen                   California Institute of Technology
> Division of BIology; 216-76     Pasadena, CA  91125
> (818) 356-6822                  chenzr at starbase1.caltech.edu