luciferin-esters and flow cytometry?

David de Graaf bmgraaf at dapsas1.weizmann.ac.il
Wed Sep 13 09:08:18 EST 1995


Dear Netters, I have heard that it is possible to assay luciferase
activity by
ordinary flow cytometry in individual cells using luciferin-esters.

Although I have found one reference, which I do not have handy, for
analyzing
luciferase expression by FACS, these good people perform the assay on sf9
insect cells. Does anyone have experience with such an assay system on
mammalian cells? Thanks in advance,

David de Graaf

Dr. David de Graaf
Department of Membrane Research and Biophysics
Weizmann Institute of Science
Rehovot, Israel 76100
tel *972 8 343 686
fax *972 8 344 112



More information about the Methods mailing list