Asymmetric PCR

AShaw5 ashaw5 at aol.com
Sat Sep 16 01:14:48 EST 1995


Yes, we use asymmetric PCR to generate probes.  It works quite well.  We
add all of the nucleotides at normal concentrations and then use cold dCTP
at 0.01X less with hot dCTP.  Cycle 30 times, check incorporation by TCA
precipitation and go.
Andrey Shaw



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