Separation dsDNA and ssDNA

ToxicTish at aol.com ToxicTish at aol.com
Fri Sep 15 08:38:46 EST 1995


Hi,

     Does anyone know of a good method to separate dsDNA and ssDNA other than
hydroxyapitite columns ?   

  Hydroxyapitite gave very poor DNA recovery.  The column was run at 60 C and
several different buffer concentrations used, but to no avail.  I need to
separate my double stranded gap heteroduplexe DNA from my left over ssDNA.
 Any suggestion appreciated but the cheaper the better.  University funds are
always low.


     Thanks


Laura Custer
Chemistry Dept.
The American University
Washington D.C.   



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