Azide inhibition of HRP

John Sargeant john01 at
Fri Sep 15 16:19:11 EST 1995

In article <amorla-1209951511110001 at>,
amorla at (Alex Morla) wrote:

> Perhaps this was unknown only to me, but today I learned (after botching
> up three experiments) that horse radish peroxidase is inhibited by azide.
> If you use azide in your wash and/or incubation buffers for western
> blotting ECL analyses you will get virtually No Signal. This isn't even
> stated in the literature from Amersham! I guess they figure nobody uses
> azide as a preservative. >:-|
> So it goes.
> -- 
> Dr. Alex Morla
> Dept. of Pathology
> The Univ. of Chicago                                              

I had never thought of that! Thanks for the observation.  Mike

m.witty at

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