mRNA isolation questions
hutchins at fiona.umsmed.edu
Fri Sep 15 12:48:37 EST 1995
Edward Wang (ez022056 at dale.ucdavis.edu) wrote:
[First question deleted because I can't answer it :-)]
: My second question is what is the best way to get mRNA into solution
: without compromising the integrity of the mRNA if
: you dried your pellet too much and it won't go into solution at normal
You can heat it to 65 deg C without harming it. 5-15 min should do it.
Be aware that most RNase inhibitors are inactivated by this treatment,
so if you use an inhibitor, you might want to add it back after heating.
Assoc Prof Anatomy, Asst Prof Neurology (Research), Univ Mississippi Med Ctr
http://fiona.umsmed.edu/~hutchins/ *** E-mail: hutchins at umsmed.edu
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