Protease deficient hosts for expression

Peter Carlsson Peter.Carlsson at
Mon Sep 18 04:26:01 EST 1995

Does anyone have/know of good E coli strains with as many as possible of 
the protease genes inactivated. The protein I´m trying to express is 
rapidly degraded in all the strains I have tried. I would preferstrains 
that are Kan-s and Amp-s and therefore compatible with the Qiagen 
His-tag system.

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