EtBr filter for CCD camera?
Christopher B. Yohn
cyohn at scripps.edu
Tue Sep 19 18:37:55 EST 1995
> : >From: Pavel Sova <ps44 at columbia.edu>
> : >Subject: EtBr filter for CCD camera?
> : >
> : >Hi there,
> : >we have a neat setup of CCD camera connected to the computer and are
> : >taking pictures of anything short of EtBr stained DNA on agarose gels.
> : >
> : >When I tried to do this, camera took very high bacground of UV bulbs,
> : >even showing some numbers written on bulbs. I wasn't able to block this
> : >background by orange filter, which we routinely use for photography of
> : >EtBr stained DNA on agarose gels (on Polaroid films). Neither UV blocking
> : >filter did any difference.
> : >
> : >I think that some of the light emitted from UV source is actually of
> : >blue color, which goes through UV blocking filter and also through orange
> : >filter. Do you have anybody suggestion what filter shoud we use (and it's
> : >number)?
> : >
CCD cameras are very sensitive to IR light, which the UV illuminator emits
plenty of. You need a good band pass filter that blocks from UV to IR.
We use one from Omega Optical [(802)254-2690]. It's a glass band pass
filter (585 +/- 35 nM) mounted in a 49 mM screw-on filter holder. It
blocks from UV to 1.2 µm and cost us $325.
Just call them up, tell them what you want and they'll make it for you and
ship it to you in a couple days. We tried a number of different filter
setups before finding this one, and this filter is great.
Christopher B. Yohn [cyohn at scripps.edu]
The Scripps Research Institute
Dept. of Cell Biology - MB8
La Jolla, CA 92037
Ph: (619) 554-4335
Fax: (619) 554-6188
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