Northern Blot - METHYLENE BLUE
hyim at u.washington.edu
Tue Sep 19 16:32:28 EST 1995
Try this reference: RNA blots: staining procedures and optimization of
conditions. Wilkinson et al. NAR 19(3):679 (1990). They used
methylene blue to stain the entire blot and claim that they got only a
30% reduction in hybridization signal after probing. Good Luck.
Harry Yim, Ph.D.
Children's Hospital and Medical Center
Department of Infectious Diseases
4800 Sand Point Way NE
Seattle, WA 98105.
On 19 Sep 1995 Dirk at .MISSING-HOST-NAME. wrote:
> We would like to establish the "intactness" of our RNA after Northern Blotting and before hybridisation with a probe.
> Is it possible to stain a Gene Image Hybound membrane with something to see the rRNA blotted? I heard something about Methylene blue or Gold Stain??
> Could this be a good method to verify the amount of RNA (in stead of using an actine probe).
> please send mail to: dirk.jochmans at chaos.kulnet.kuleuven.ac.be
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