purification of protein from exclusion bodies

[Jennifer Lyon]

Hello,

"Methods in enzymology" Volume 182 is a very good reference
 for protein purification. For inclusion bodies, see chapter
20:  Marston and Hartley, "Methods in Enzymology" 182:164-276.

Also, here are a few references involving insoluble proteins
produced with pET vectors:
1. McCarty, et al. 1991. Cell 66:895-905.
2. Lathrop, et al. 1992. Prot.Exp.&Purif. 3:512-517
3. Fischer, et al. 1992. 257:1392-1395.
4. Young, et al. 1994. Cell 76:39-50
5. Tabata and Kornberg. 1994. Cell 76:89-102

Hope this is of some help.
-Jennifer Lyon
JLyon at Novagen.com

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