Two questions

Igor R Sagdeev isagdeev at osf1.gmu.edu
Tue Sep 19 10:37:18 EST 1995


Dear netters,

I have two questions for those who have any experience in these two
things:

1. What are the easiest and most efficient ways of getting rid of
   unincorporated primers and nucleotides after a PCR without losing
    too much of the product?

2. what is the simplest protocol for TCA or HCl precipitation to
   assess incorporation of label in generation of radiolabeled
   probes?

						Yours, Igor




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