sequencing pcr product with 35S

Igor R Sagdeev isagdeev at osf1.gmu.edu
Tue Sep 19 10:33:27 EST 1995


In article <alshiau.43.005C5990 at mail.ncku.edu.tw> alshiau at mail.ncku.edu.tw (Ai-Li Shiau) writes:
>Hi Netter,
>
>Is there anybody having the experience with sequencing PCR product using 
>35S-dATP either end-labelling or direct incorporation ?  Any detail protocol 
>or suggestion  would be appreciated. 
>*******************************************************************************************
>   Ai-Li Shiau,Ph.D.                    EMail:  alshiau at mail.ncku.edu.tw
>   Department of Microbiology          
>   Medical College                      Phone:  +886-6-2353535 ext 5629
>   National Cheng Kung University       Fax:    +886-6-2082705 
>   Tainan, Taiwan                               +886-6-2363715
>*******************************************************************************************
>
Buy Sequenase 2.0 sequencing kit from Amersham. It does exactly that
through 2-step labelling/extension protocol.
					Yours, Igor

>





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