freeze-drier for HPLC fractions

Wolfgang Schechinger u7k0201 at sunmail.lrz-muenchen.de
Tue Sep 19 08:27:30 EST 1995


Denni Schnapp <ds4 at st-andrews.ac.uk> wrote:
Hi Denni!

We are using a cold trap (about -50°C) before the vacuum pump to freeze out solvents like 
chloroform.

It might be suitable for TFA and ACN, too. In addition, you also could use another tube filled 
with sodium carbonate to absorb leftover TFA vapors and/or a tube filled with a suitable 
absorbant for ACN.

Good luck! 
Wolfgang 



>Dear netters,
>
>We are doing a lot of reverse phase HPLC separation of peptides
>with ACN gradients in 0.1% TFA. We need to freeze-dry our HPLC
>fractions and are currently thinking about obtaining our own
>freeze-drying system. Several people have told me that neither ACN
>nor TFA are particularly good for the pump. So, does anyone know
>about what type of (cheap!) pump may be best suited for the job?
>Any experiences?
>
>Thanks for your advise!
>
>
><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<
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>
>Denni Schnapp		    
>Gatty Marine Laboratory    			Fax: (0)1334-463443
>University of St. Andrews			e-mail: ds4 at st-and.ac.uk
>St. Andrews Fife KY16 8LB   			
>Scotland		   	http://www.st-and.ac.uk/~www_sa/personal/ds4/
>		     
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><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<

-- 
+++++++++++++++++++++++++++++++++++++++++++
Wolfgang Schechinger
Institute for Diabetes Reserch 
Koelner Platz 1
80804 Munich
Germany 
Phone +49 (89) 30 79 31 24
Fax   +49 (89) 30 81 733
email u7k0201 at sunmail.lrz-muenchen.de

(Standard disclaimer applies)
+++++++++++++++++++++++++++++++++++++++++++





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