EtBr filter for CCD camera?
jtudor at sju.edu
Tue Sep 19 06:45:50 EST 1995
Check with a company that sells gel documentation systems, such
as Stratagene, UVP, Fotodyne, etc. I have a Stratagene unit and
they sell a filter for the CCD camera that works very well for
EtBr stained gels illuminated using a transilluminator.
Hemant Chickarmane (hemant at hoh.mbl.edu) wrote:
: In article writes:
: >From: Pavel Sova <ps44 at columbia.edu>
: >Subject: EtBr filter for CCD camera?
: >Hi there,
: >we have a neat setup of CCD camera connected to the computer and are
: >taking pictures of anything short of EtBr stained DNA on agarose gels.
: >When I tried to do this, camera took very high bacground of UV bulbs,
: >even showing some numbers written on bulbs. I wasn't able to block this
: >background by orange filter, which we routinely use for photography of
: >EtBr stained DNA on agarose gels (on Polaroid films). Neither UV blocking
: >filter did any difference.
: >I think that some of the light emitted from UV source is actually of visible
: >blue color, which goes through UV blocking filter and also through orange
: >filter. Do you have anybody suggestion what filter shoud we use (and it's
: A simple solution would be to irradiate the gel from the side like so:
: UV source
: This would eliminate direct UV from getting to the camera.
: The standard screw-on orange filter would suffice.
: Hope this helps. Good luck.
: Dr Hemant Chikarmane, Marine Biological Laboratory, Woods Hole, MA 02543 USA
: email: hemant at mbl.edu voice: (508) 289-7245
: **** Experience Fahrvergnuegen - Drive a Macintosh ****
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