EtBr filter for CCD camera?

[John Tudor]

Check with a company that sells gel documentation systems, such
as Stratagene, UVP, Fotodyne, etc.  I have a Stratagene unit and
they sell a filter for the CCD camera that works very well for
EtBr stained gels illuminated using a transilluminator.

Hemant Chickarmane (hemant at hoh.mbl.edu) wrote:
: In article   writes:
: >From: Pavel Sova <ps44 at columbia.edu>
: >Subject: EtBr filter for CCD camera?
: >
: >Hi there,
: >we have a neat setup of CCD camera connected to the computer and are 
: >taking pictures of anything short of EtBr stained DNA on agarose gels.
: >
: >When I tried to do this, camera took very high bacground of UV bulbs, 
: >even showing some numbers written on bulbs.  I wasn't able to block this 
: >background by orange filter, which we routinely use for photography of 
: >EtBr stained DNA on agarose gels (on Polaroid films).  Neither UV blocking 
: >filter did any difference.
: >
: >I think that some of the light emitted from UV source is actually of visible 
: >blue color, which goes through UV blocking filter and also through orange 
: >filter.  Do you have anybody suggestion what filter shoud we use (and it's 
: >number)?
: >

: A simple solution would be to irradiate the gel from the side like so:


:                           Camera
:                           |||||| 
:                                       UV source
:                                      ////
:                                     ////
:                           ======= 
:                             Gel

: This would eliminate direct UV from getting to the camera.
: The standard screw-on orange filter would suffice.

: Hope this helps.  Good luck.


: -- 
: Dr Hemant Chikarmane, Marine Biological Laboratory, Woods Hole, MA 02543 USA
:            email: hemant at mbl.edu	voice: (508) 289-7245

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