Using Pwo DNA polymerase for blunting.

Aart A. van Apeldoorn A.A.vanApeldoorn at med.ruu.nl
Wed Sep 20 07:11:56 EST 1995


I'm using Pwo DNA polymerase for blunting of sticky ends, instead of
klenow. Did anyone do this before and what are your experiences with this
PCR enzyme ?. I'm using 2.5 units in a 20 ul volume, using 200 uM dNTP
concentration, do you have any interesting suggestions.

-- 
Aart A. van Apeldoorn
Dept. of Medical Physiology & Sports Medicine
Faculty of Medicine, Utrecht University
MFU-2 "Stratenum"
Universiteitsweg 100
3584 CG Utrecht
The Netherlands
E-mail: A.A.vanApeldoorn at med.ruu.nl



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