Problem with REP and ERIC primers

TAN HUI HUI sci20398 at leonis.nus.sg
Tue Sep 19 05:03:12 EST 1995


 Recently , I bought a set of REP and ERIC primers which are supposed to 
amplified repetitive sequences of Gram negative bacteria. 

But , when I did PCR with either of them, I did not get any expected 
results which are supposed to show several bands distinct for EACH type 
of bacteria.

So, what could be my problem? I have already tried optimizing using 
different conditions but I always end up with either NO bands or just a 
smear on a 1% agarose gel.

Does the problem lie in the primers?


Please enlighten me!

GINA.



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