Problem with REP and ERIC primers
TAN HUI HUI
sci20398 at leonis.nus.sg
Tue Sep 19 05:03:12 EST 1995
Recently , I bought a set of REP and ERIC primers which are supposed to
amplified repetitive sequences of Gram negative bacteria.
But , when I did PCR with either of them, I did not get any expected
results which are supposed to show several bands distinct for EACH type
So, what could be my problem? I have already tried optimizing using
different conditions but I always end up with either NO bands or just a
smear on a 1% agarose gel.
Does the problem lie in the primers?
Please enlighten me!
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