Blunt lig. help (NL)

Jillian Johnston johnstoj at acs2.acs.ucalgary.ca
Thu Sep 21 14:34:06 EST 1995


Are you sure the enzyme is good? Is the concentration of ATP high
enough?  We aliquot the buffer with ATP because we ahve found ATP
to degrade after a few freeze/thaws.  Do you add PEG8000 to a
final conc. of 13%? We use a 40% stock.
Good luck!
Jill



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